Subject: Analytical Biochemistry II

Scientific Area:



68 Hours

Number of ECTS:




Overall objectives:

1 - The objective of this course is to introduce the hyphenated and spectroscopic chromatographic methods (theoretical concepts and applications) used in the biochemical, structural and spectroscopic characterization of biological systems. With a strong experimental component, with an environment very similar to that of a research laboratory, it is intended that students:
1.1 - Develop the ability to understand and select appropriate techniques and methodologies for studying a particular biological system
1.2 - Cultivate a critical spirit in analyzing and interpreting in an integrated way the results obtained
1.3 - Recognize the fundamentals of hyphenated and spectroscopic chromatographic techniques and their importance in biomolecule analysis.
2 - The writing and discussion of a final report aims to familiarize students with scientific writing and acquire training in critical review of scientific literature and skills in presenting scientific papers.


0 - The syllabus of the curricular unit includes the following subjects:
1 - Analysis and quantification of biomolecules:
1.1 - Strategies used in protein isolation, purification and analysis. Experimental strategy to use versus study objective
1.2 - Carbohydrate (HC) analysis methods: Chemical and enzymatic methods used for HC detection and quantification
1.3 - Separation and identification of HS mixtures
1.3.1 - Lipid analysis methods: Sample preparation; Quantitative methods; Separation of lipid mixtures.
2 - Tandem mass spectrometry (GC-MS and LC-MS): Theoretical principles; applications
3 - NMR bioanalysis: theoretical principles; Biochemistry Applications
4 - Validation of new bioanalytical methods - determination of the merit figures of the analytical method
i - Main methods used in biomolecule analysis;
i.2 - Qualitative analysis vs quantitative analysis;
i.3 - Parameters used in the identification and quantification of biomolecules.


David Holme; Hazel Peck, Eds , 1998 , Analytical biochemistry , Prentice Hall Publisher
Susan R. Mikkelsen, Eduardo Corto´n, Eds , 2004 , Bioanalytical chemistry - Susan R. Mikkelsen, Eduardo Corto´n, Eds , John Wiley & Sons, Inc., Hoboken
Andreas Manz, Nicole Pamme, Dimitri IossifidisI, Eds , 2004 , Bioanalytical chemistry , Imperial College Press
Victor A. Gault; Neville H. McClenaghan , 2009 , Understanding bioanalytical chemistry. Principles and applications , John Wiley & Sons Inc,

Assesssment methods and criteria:

Classification Type: Quantitativa (0-20)

Evaluation Methodology:
The teaching/learning activities are based on several approaches, including lectures and practical classes focused on interpretation, discussion and problem solving that aim to deepen the concepts presented in lectures. In PL classes, experimental works are carried out to illustrate some of the themes developed in theoretical classes, whose results and conclusions are the subject of oral discussion. Only students who have attended the course will be admitted for assessment T: compulsory attendance of all PL and group report submission and discussion (1 / group) PL evaluation: i) continuous laboratory evaluation (punctuality, preparation and performance in the experimental execution); ii) Evaluation of the report and its discussion Assessment T: 2 theoretical tests, with a weight of 35% each AF = 70% T + 30% PL Passing the course requires a grade of 9.5 in each of the components, T and PL