Subject: Laboratory Techniques II

Scientific Area:



94 Hours

Number of ECTS:




Overall objectives:

1.1 - Acquire basic knowledge about simple techniques to use across all units of the course either in terms of understanding of the phenomena that are at the base of these techniques, or their practical application
1.2 - Learn and apply concepts of safety in the laboratory
1.3 - Acquire skills in handling material and equipment
2.1 - To recognize and learn to handle basic laboratory equipment
2.2 - To be able to calibrate and use lab equipment and make sense of the obtained data
2.3 - To develop concentration, critical spirit and autonomy
2.4 - To work as a team
2.5 - To relate the knowledge acquired from the phenomena observed in everyday life
2.6 - To plan, execute, and propose strategies, interpret experimental results and communicate them


1 - Volumetry, Condutimetria and Potentiometry
2 - Volumetry based in neutralization, precipitation, oxi-complexation and reduction. Buffer solutions. Conductimetry
3 - Types of electrodes. Potenciometric titrations
4 - Interaction of radiation with matter
5 - Electromagnetic spectrum. Types of radiation. Ionization and excitation. Applications in diagnostic procedures and medical therapy
6 - UV/Vis spectrophotometry
7 - Concepts and terms used in absorption spectroscopy. Lambert-Beer law. Molecular absorption spectroscopy of organic molecules and inorganic species. Qualitative analysis and quantitative analysis.
8 - Molecular absorption spectroscopy
9 - Organic molecules and inorganic species. Qualitative Analysis and Quantitative Analysis
10 - Infrared spectroscopy
11 - Theory of infrared absorption. Specimen handling techniques. Acquisition of Spectra. Qualitative applications. General principles of Chromatography
12 - General principles of Chromatography
13 - Mobile and stationary phases. Types of chromatography, separation principles and experimental media. Eluotropic series. Stains. Applications in biochemistry.
14 - Applications in biochemistry.


Skoog, West and Koller , Analytical Chemistry , Saunders College Publishing
Pombeiro, A. J. L. O. , 2003 , Técnicas e Operações Unitárias em Química Laboratorial Edição/reimpressão , Fundação Calouste Gulbenkian
Castilho, Paula; Cordeiro, Nereida; Câmara, José S. , 2014 , Técnicas Laboratoriais Elementares , s/ed

Assesssment methods and criteria:

Classification Type: Quantitativa (0-20)

Evaluation Methodology:
This UC comprises of 24 hours of lectures spread over 16 weeks and 70 h of lab practice, spread over 14 weeks (2 blocks of 3 hours per week). In the theoretical classes, the professor makes use of multimedia projection, white board and demonstration with various materials. The practical classes are laboratory sessions where groups of students apply the techniques under study to concrete cases. The filling in of post-lab questionnaires ("reports") is made at the end of each session or group of sessions Evaluation in this UC consists of: a) written tests (2) weight of 30%); The minimum score on each test is of 8 values. b) continuous evaluation in the practical classes, based on the development and delivery of questionnaires, preparations of each work, performance during sessions, elaboration and timely delivery of reports and response to proposed application exercises. All these elements are grouped in the Lab Manual (weight of 70%).