Subject: Analytical Biochemistry I

Scientific Area:



60 Hours

Number of ECTS:




Overall objectives:

1 - To acquire a general knowledge of analytical chemistry, with emphasis on the instrumental analysis.
2 - To understand the current use of spectrophotometric and chromatographic methodologies, as well as the equipment and terminology.
3 - To develop the concept of the potential of separation techniques, identification and determination of components in complex mixtures.
4 - Training and planning for team work and development of critical thinking and how to communicate results.


1 - Introduction to analytical biochemistry
1.1 - Fundamental Concepts; Implementation of methodologies; Quality assurance
2 - Molecular Spectroscopy
2.1 - Concepts; Beer-Lambert Law; UV-vis spectrophotometry; IR spectrophotometry; applications
3 - Atomic Spectroscopy
3.1 - Concepts; fundamentals of atomic absorption; applications
3.2 - ICP-AES (Inductively Coupled Plasma - Atomic Emission Spectrometry): Concepts and applications
4 - Separation Techniques
4.1 - Chromatographic principles and separation factors; chromatographic separation measurements and models
4.2 - Chromatographic Separation Methods
4.3 - Gas chromatography: principles and applications
4.4 - Liquid chromatography: principles and applications
5 - Introduction to hyphenated techniques
5.1 - Principles, interfaces and applications


D.A. Skoog, F. J. Holler, S. R. Crouch , 2006 , Principles of Instrumental Analysis , Thompson Brooks/Cole
H. Wilard, L. Merrit Jr., J. Dean , Análise Instrumental ,
H.J. Chaves das Neves , Introdução à Pratica da cromatografia gas-líquido , Universidade Nova de Lisboa
José Carlos Antunes Marques , Documentação disponibilizada pelo docente / documentation supplied by the teacher. ,

Assesssment methods and criteria:

Classification Type:

Evaluation Methodology:
The course comprises 38 hours of lectures and 22 hours of practical classes. Theoretical teaching is accompanied by multimedia projection and the discussion of practical cases. The laboratory classes involve learning / training laboratory techniques used in spectrophotometric analysis and chromatographic separations. Basic information for the completion of work is supplied to the students but the group should plan the work to be done in the laboratory. Students are also challenged to do a study of their own interest and on their own proposal in order to stimulate the application of their own skills and interests to the area. The evaluation of this course consists of: a) written tests to assess the knowledge and skills acquired by each student (50% weight); b) reports of the experimental work (30% weight); c) work of the students initiative and participation (20% weight).